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The Green Mother Machine project folder

All documents and images for the project

The Green Mother Machine: a microfluidics device for cyanobacteria

Optimising a microfluidic chemostat for experiments using cyanobacteria, including growth and feeding channels.

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The Green Mother Machine: A microfluidics device for cyanobacteria full application

Cyanobacteria are ubiquitous autotrophic bacteria that have the natural ability to utilize sunlight to convert atmospheric CO2 into carbohydrates. Cyanobacteria’s photosynthetic abilities are very appealing to synthetic biology and biotechnology. Microfluidic chemostats provide an ideal environment in which the environmental conditions can be controlled tightly. The media can be exchanged easily during the experiment thus allowing for switching between for example carbon rich and carbon poor media or media with and without specific stress inducers. The mother machine design has the following advantages compared to other chemostats: 1. The mother cell can be followed for an unlimited period of time. 2. As the cells grow in a line, rather than randomly orientated like in the case of a normal bacteria colony, image analysis (segmenting and single-cell tracking) is easier. 3. The media can still be easily exchanged.

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The Green Mother Machine

The Green Mother Machine - a microfluidics device for cyanobacteria (.pdf)

The Green Mother Machine - Read More…

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The Synthetic Biology Strategic Research Initiative provides a hub for anyone interested in Synthetic Biology at the University of Cambridge, including researchers, commercial partners and external collaborators. 

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